Subunit Recycling during Translation in a Reticulocyte Cell-free System

Protein synthesis in a cell free human reticulocyte system: ribosome function in thalassemia.

Synthesis of rat liver albumin in a rabbit reticulocyte cell-free protein-synthesizing system.

Rat liver polysomal RNA can direct the synthesis of albumin in a cell-free system derived from rabbit reticulocytes. The in uifro product, labeled with [3H]leucine, was identified as albumin by precipitation with specific antibody and by comparison to authentic serum [Wlalbumin. The in uifro product and serum albumin co-migrate during electrophoresis in sodium dodecyl sulfate polyacrylamide gel...

Inhibitory effect of alpha-fetoprotein on protein synthesis in a reticulocyte lysate cell-free system.

The inhibitory effect of human alpha-fetoprotein on the protein synthesis (incorporation of [3H]leucine into the total protein fraction) weas observed in a rabbit reticulocyte lysate cell-free system. The incorporation was inhibited to 50% of the control level by 14-16 microM alpha-fetoprotein, and decreased to about 15% at a concentration higher than 40 microM. An almost identical dose depende...

Translation of liver messenger ribonucleic acid in a messenger-dependent reticulocyte cell-free system. Properties of the system and identification of ferriitin in the product.

An RNA fraction with messenger activity has been isolated from free polysomes of rabbit liver. This liver RNA was translated in a reticulocyte cell-free system, which had been rendered dependent on exogenous messenger RNA by mild ribonuclease treatment. After ribonuclease was removed from the ribosomes, protein synthesis was almost totally dependent on 0.5 M KC1 ribosomal wash protein and exoge...

investigating the integration of translation technologies into translation programs in iranian universities: basis for a syllabus design in translation technology

today, information technology and computers are indispensable tools of any profession and translation technologies have become an indispensable part of translator’s workstation. with the increasing demands for high productivity and speed as well as consistency and with the rise of new demands for translation and localization, it is necessary for translators to be familiar with market demands an...